Light Sheet Microscopy

This volume provides researchers with a comprehensive guide for understanding light-sheet fluorescence microscopy (LSFM), and covering issues in both its routine use of the method and the latest advancements. The chapters in this book are organized into three parts: Part One is an introduction to the method, its history, and a review on tissue clearing. Part Two focuses on recent advancements in LSFM that cover increasing resolution, field of view, and image contrast. Part Three presents several guides to the use of LSFM in an imaging facility, human brain imaging, mapping of immediate early genes, and in vivo functional imaging on Zebrafish. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and thorough, Light-Sheet Microscopy is a valuable resource for all researchers who are interested in learning more about the basic and advanced uses of LSFM.