Protein Evolution

This volume details computational tools for the study of protein evolution based on 1D-sequence and 3D-structure databases. Chapters guide readers through the analysis of 1D protein sequences for coevolutionary fold prediction, protein-nucleic acid interactions, distant homologs and functional motifs, characterization of fold energetics, disordered proteins, mutagenesis-driven modulation of conformational landscapes, 3D protein domains, prediction of 3D protein-protein interactions, and hybrid methods that mine both 1D and 3D genomic and protein databases for gene editing and alternative splicing. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Evolution: Methods and Protocols aims to be comprehensive guide for researchers in the field.