Laboratory Exercises In Microbiology Holec-Gąsior
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LABORATORY EXERCISES IN MICROBIOLOGY
Lucyna Holec-Gąsior, Katarzyna Werbowy, Józef Kur
Stan książki: NOWA
Wydawnictwo Politechniki Gdańskiej
Stron:106
Format: B5
Słowa kluczowe: ćwiczenia z mikrobiologii, mikrobiologia, laboratorium
Spis treści:
PREFACE 5
1. STERIIJZATIONANDDISINFECTION 7
1.1. Musical sterilization 7
1.2. Chemical agents. 13
2. CULTIVATION OF MICROORGANISMS 21
2.1. Growth curve .. 21
2.2. Culture media . 22
2.3. Nutritional requirements of cells .. 25
2.4. Microorganisms in the laboratory en\ ironment 20
2.4.1. Colony morphology 26
2.4.2. Growth patterns in broili, agar siani and agar deep-tube. 27
2.5. Pure culture techniques.. 31
2.5.1. Spread plate technique^1
2.5.2. Streak plate technique ^2
2.6. Specific media .$6
3. ENVIRONMENTAL FACTORS AFFECTING MICROBIAL GROWTH 41
3.1. Temperaturę. 41
3.1.1. Effects on growth 41
3.1.2. Lethal effects . 42
3.2. pil . 44
3.3. Osmotic pressure .. 46
3.4. Ultraviole1 lighl - lethal effects . 48
3.5. Oxygen requirements cultivation of anaerobes . 50
4. ENUMERATION OF BACTHRIA 54
5. ANTIMICROBIAl AGENTS . 59
6. METABOLIC ACTIVITES OF MICROORGANISMS 65
6.1. Hydrolysis of siar eh . 66
6.2. Hydrolysis of lais.. 67
6.3. Hydrolysis of proteins 68
6.4. Casein hydrolysis.. 70
6.5. Fermentation and p-galactosidase acii\ ity .. 71
7. MICROBIALRELATIONSHIPS.. 76
7.1. Bacterial commensalism . 76
7.2. Bacterial synergism 76
7.3. Microbial antagonism .. 77
8. MICROSCOPIC TECHNIQUES AND BACTERIA STAINING <SI
8.1. Microscopy SI
8.2. Microscope slide techniąue - bacteria staining .. 84
8.3. Gram staining 87
9. IDENTIFICATION OF UNKNOWN BACTERIA I ROM ENVIRONMENTAL SAMPLES .. l)|
10. MICROBIAL GENETICS . 97
10.1. Izolation of plasmid dna.. l)7
10.2. Gel electrophoresis. 99
10.3. Bacterial transformation.. 101
REFERENCES.. 105